Structured Bilaminar Coculture Outperforms Stem Cells and Disc Cells in a Simulated Degenerate Disc Environment
نویسندگان
چکیده
منابع مشابه
Structured coculture of mesenchymal stem cells and disc cells enhances differentiation and proliferation.
PURPOSE During in vivo stem cell differentiation, mature cells often induce the differentiation of nearby stem cells. Accordingly, prior studies indicate that a randomly mixed coculture can help transform mesenchymal stem cells (MSC) into nucleus pulposus cells (NPC). However, because in vivo signaling typically occurs heterotopically between adjacent cell layers, we hypothesized that a structu...
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BACKGROUND Our goal is to optimize stem cell-based tissue engineering strategies in the context of the intervertebral disc environment. We explored the benefits of co-culturing nucleus pulposus cells (NPC) and adult mesenchymal stem cells (MSC) using a novel spherical bilaminar pellet culture system where one cell type is enclosed in a sphere of the other cell type. Our 3D system provides a str...
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General Abstract Current treatments for the degenerated intervertebral disc (IVD) comprise pain management and invasive surgical interventions; both of them are aimed at relieving the pain instead of repairing the tissue and restoring the biological function of the disc. Cell-based tissue engineering may meet the clinical need for the repair or regeneration of a damaged tissue, and it might be ...
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The disintegration of charged alkaline mercury button cells in simulated gastric fluid over a 24 h period has been studied. The integrity of the cells and the amount of mercury that can leak out of them were assessed. The cells raised the pH of the incubating solutions. Disruption was seen in five out of 18 cells tested in 0.1 mol/L hydrochloric acid and one out of nine in 0.9% saline. Five of ...
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Low back pain secondary to degenerative intervertebral disc disease contributes to significant pain and disability in the United States. The potential to reverse intervertebral disc degeneration has driven significant interest in the development of stem cell therapy for degenerative disc disease. Early animal models have demonstrated that stem cell therapy may facilitate the reversal of the dis...
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ژورنال
عنوان ژورنال: Spine
سال: 2012
ISSN: 0362-2436
DOI: 10.1097/brs.0b013e31823b055f